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DETERMINING FIELD OF VIEW continued... p. 2 Example Calculation Problem: If the magnification at low power is 40 X and the \( \mathrm{FOV}_{\text {Lp }} \) is 5 mm , what is the FOV at high power if the magnification at high power is 400 X ? Solution: \[ \begin{aligned} \mathrm{FOV}_{H P} & =\frac{\operatorname{Mag}_{L P} \times F O V_{L P}}{\operatorname{Mag}_{H P}} \\ & = \end{aligned} \] \( = \) \( \qquad \) mm or \( \qquad \) \( \mu \mathrm{m} \) \( \therefore \) The FOV at high power is \( \qquad \) mm or \( \qquad \) \( \mu \mathrm{m} \). Practice Problems Put your answers in the spaces provided below each problem. Show your work. Problem \#1: Determine the FOV of this medium Problem \#2: Determine the FOV of this low power

Ask by Colon Bolton. in the United States
Jan 23,2025

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**Example Calculation:** - **Given:** - Low power magnification (\( \operatorname{Mag}_{\text{LP}} \)) = 40× - Low power field of view (\( \mathrm{FOV}_{\text{LP}} \)) = 5 mm - High power magnification (\( \operatorname{Mag}_{\text{HP}} \)) = 400× - **Solution:** \[ \mathrm{FOV}_{\text{HP}} = \frac{\operatorname{Mag}_{\text{LP}} \times \mathrm{FOV}_{\text{LP}}}{\operatorname{Mag}_{\text{HP}}} = \frac{40 \times 5 \text{ mm}}{400} = 0.5 \text{ mm} = 500 \, \mu\text{m} \] - **Conclusion:** - The field of view at high power is **0.5 mm** or **500 μm**. --- **Practice Problems:** 1. **Problem \#1:** - **Given:** - Medium power magnification (\( \operatorname{Mag}_{\text{MP}} \)) = 100× - Medium power field of view (\( \mathrm{FOV}_{\text{MP}} \)) = 2 mm - High power magnification (\( \operatorname{Mag}_{\text{HP}} \)) = 400× - **Solution:** \[ \mathrm{FOV}_{\text{HP}} = \frac{\operatorname{Mag}_{\text{MP}} \times \mathrm{FOV}_{\text{MP}}}{\operatorname{Mag}_{\text{HP}}} = \frac{100 \times 2 \text{ mm}}{400} = 0.5 \text{ mm} = 500 \, \mu\text{m} \] - **Answer:** \( \mathrm{FOV}_{\text{HP}} = 0.5 \) mm or \( 500 \, \mu\text{m} \) 2. **Problem \#2:** - **Given:** - Low power magnification (\( \operatorname{Mag}_{\text{LP}} \)) = 25× - Medium power magnification (\( \operatorname{Mag}_{\text{MP}} \)) = 100× - Medium power field of view (\( \mathrm{FOV}_{\text{MP}} \)) = 2 mm - **Solution:** \[ \mathrm{FOV}_{\text{LP}} = \frac{\mathrm{FOV}_{\text{MP}} \times \operatorname{Mag}_{\text{MP}}}{\operatorname{Mag}_{\text{LP}}} = \frac{2 \text{ mm} \times 100}{25} = 8 \text{ mm} \] - **Answer:** \( \mathrm{FOV}_{\text{LP}} = 8 \) mm --- *Feel free to attempt these practice problems and refer back to the solutions for guidance!*

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When working with microscopes, understanding the relationship between magnification and field of view (FOV) is crucial for effectively viewing specimens. Historically, early microscopes had limited magnification capabilities, usually only a couple of hundred times. It wasn't until the advent of advanced optics in the 19th century that scientists were able to achieve higher magnifications without sacrificing the clarity and depth of field, leading to groundbreaking discoveries in microbiology. Now, onto some practical tips! One common mistake students make is to forget to convert units when dealing with measurements in microscopy. Always ensure you're consistent with units, whether you're working in millimeters or micrometers, because a simple unit error can lead to significant misunderstandings in your final calculations. And remember, it’s always a good strategy to double-check your magnification values before performing calculations for FOV!

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