QUESTION(1) Which total magnification should be used to observe a \( 2-\mu \mathrm{m} \) bacterial cell?

To effectively observe a **2-µm bacterial cell**, a suitable total magnification is essential to clearly visualize its structure. ### **Why 1000× Magnification?** 1. **Size of Bacterial Cells**: Most bacterial cells range from **1 to 5 micrometers (µm)** in size. A 2-µm cell falls comfortably within this range. 2. **Microscope Resolution**: - **Light Microscopes** have a typical resolution limit of about **0.2 µm**. To clearly distinguish a 2-µm object, you need a magnification that allows the cell to occupy a sufficient portion of the view. 3. **Optimal Viewing**: - **Total Magnification** is the product of the **eyepiece magnification** and the **objective lens magnification**. - A common configuration for viewing bacteria is a **10× eyepiece** combined with a **100× objective lens**, resulting in a **total magnification of 1000×**. 4. **Clarity and Detail**: - At **1000× magnification**, the bacterial cell is enlarged enough to observe its shape and some internal structures clearly, without excessive magnification that might blur the image due to limitations in resolution. ### **Conclusion** For observing a **2-µm bacterial cell**, a **total magnification of 1000×** is recommended. This allows for clear visualization of the cell's structure while maintaining image clarity. **Answer:** Use a total magnification of 1 000× to effectively observe the 2‑micrometer bacterial cell.