Newton Weston
03/10/2023 · Primary School

Total dilution of \( 3^{\text {rd }} \) plate \( =1: 1000 \) Concentration of Original Tube \( =\frac{\text { Colonies counted } \times \text { Dilution }}{\text { Volume plated }} \) Concentration of Original Tube \( =\frac{250 \mathrm{CFU} \times 1000}{0.1 \mathrm{~mL}} \) Concentration of Original Tube \( =2,500,000 \mathrm{CFU} / \mathrm{mL} \)

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The concentration of the original tube is 2,500,000 CFU/mL.

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