What is the primary role of the citric acid cycle in energy production? breaking down glucose molecules loading up shuttle buses with protons recycling citrate producing \( \mathrm{CO}_{2} \)

(B) State TWO ways in which flagellum helps a microorganism survive in an open environment. (2 ma All rights reserved. No part of this publication may be reproduced, stored in a retrieval sys transmitted in any form or by any means, electronic, mechanical, photocopying, reco otherwise, without the prior written permission of the Institute of Technical Education, Singapo Institute of Technical Education has made every effort to trace copyright holders, but if inadvertently overlooked any, we will be pleased to make the necessary arrangements a

21 When conducting the bioluminescence assay, a false positive result is detected if the sample (A) contains a lot of fat. (B) comprises of artificial dye. (C) has high initial microbial load. (D) is contaminated with chemicals. The function of cell wall in bacteria is to (A) store nutrients. (B) protect them against drying. (C) control the reproduction rate. (D) maintain the characteristic shape.

Calculate the cfu/cm \( \mathrm{cm}^{2} \) for a tested surface of 4 cm in length and 3 cm in width if the number of colonies on the agar plate is 96 cfu. (A) \( 8 \mathrm{cfu} / \mathrm{cm}^{2} \) (B) \( 12 \mathrm{cmu} / \mathrm{cm}^{2} \). (C) \( 72 \mathrm{cmu} / \mathrm{cm}^{2} \) (D) \( 128 \mathrm{cmu} / \mathrm{cm}^{2} \)

What one of the following are the CORRECT operating conditions when autoclaving biohazard wastes? (A) \( 10 \mathrm{psi} 121^{\circ} \mathrm{C} \) for 15 minutes. (B) \( 10 \mathrm{psi}, 121^{\circ} \mathrm{C} \) for 30 minutes. (C) \( 15 \mathrm{psi}, 121^{\circ} \mathrm{C} \) for 15 minutes. (D) \( 15 \mathrm{psi}, 121^{\circ} \mathrm{C} \) for 30 minutes. After conducting microbiology experiment, a laboratory technician is required to dispose the remaining culture broths. Which one of the following is the CORRECT disposal method? (A) Incineration. (B) Discard as normal waste. (C) Collection by licensed waste collectors. (D) Autoclave before discarding into the drain.

23 Which one of the following microorganisms moves using flagellum? (A) Amoeba. (B) Euglena. (C) Paramecium. (D) Plasmodium. Which one of the following is NOT a heterotrophic microorganism? \( \begin{array}{ll}\text { (A) } \quad \text { Diatoms. } \\ \text { (B) } \quad \text { Sarcodines. } \\ \text { (C) } \quad \text { Sporozoans. } \\ \text { (D) }\end{array} \)