Alejandra y Jazmín son hermanas que estudian el primer y segundo año de secundaria respectivamente en una escuela pública del estado de Veracruz El lunes pasado acudió un módulo de salud pública a la escuela para aplicar la vacuna del virus de papiloma humano, pero se le negó el derecho a la vacuna a ambas hermanas, debido a que la enfermera notó que ellas tenín los medios económicos para aplicarse la vacuna en un hospital privado. Identifica el principio de la bioética que incumple la enfermera. (A) Ninguna de las dos opciones (B) Jutonomía (C) Justicia

(A) (i) Describe TWO disadvantages of using Replicate Organism Detection And Counting (RODAC) plate during a surface swab. (ii) Explain TWO purposes of conducting a hand swab test. 12 m

(B) State TWO ways in which flagellum helps a microorganism survive in an open environment. (2 ma All rights reserved. No part of this publication may be reproduced, stored in a retrieval sys transmitted in any form or by any means, electronic, mechanical, photocopying, reco otherwise, without the prior written permission of the Institute of Technical Education, Singapo Institute of Technical Education has made every effort to trace copyright holders, but if inadvertently overlooked any, we will be pleased to make the necessary arrangements a

(A) State ONE example of selective media that can be used to isolate [3 marks] EACH of the following microorganisms. (i) Gram positive bacteria. (ii) Staphylococci spp. (iii) Gram negative bacteria.

Calculate the cfu/cm \( \mathrm{cm}^{2} \) for a tested surface of 4 cm in length and 3 cm in width if the number of colonies on the agar plate is 96 cfu. (A) \( 8 \mathrm{cfu} / \mathrm{cm}^{2} \) (B) \( 12 \mathrm{cmu} / \mathrm{cm}^{2} \). (C) \( 72 \mathrm{cmu} / \mathrm{cm}^{2} \) (D) \( 128 \mathrm{cmu} / \mathrm{cm}^{2} \)

(B) (i) Explain THREE precaution steps during decontamination of biohazard wastes in bag using an autoclave. (ii) Describe TWO materials that will remain undamaged after autoclaving.