Pregunta
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(d) Draw a labelled diagram showing the DNA replication process. Show the different
enzymes and proteins involved in the process.

Ask by Klein Long. in the United States
Mar 20,2025

Solución de inteligencia artificial de Upstudy

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Here’s a simplified diagram of the DNA replication process:
Replication Fork
│
Helicase unwinds DNA
│
SSB proteins stabilize single strands
│
Primase synthesizes RNA primers
│
DNA Polymerase III extends DNA
Leading Strand: Continuous synthesis
Lagging Strand: Synthesizes Okazaki fragments
│
RNA primers removed and gaps filled by DNA Polymerase I
│
Fragments joined by DNA Ligase
This shows the main enzymes and steps involved in DNA replication.

Solución

                         Replication Fork
                               │
                 ┌─────────────┴─────────────┐
                 │                           │
           (SSB proteins)               (SSB proteins)
               bind to                    bind to
          single-stranded DNA         single-stranded DNA
                 │                           │
                 │                           │
            ┌────┴────┐                ┌─────┴─────┐
            │ Helicase│                │ Helicase  │
            └────┬────┘                └─────┬─────┘
                 │                           │
           Unwinds the DNA            Unwinds the DNA
                 │                           │
         ┌───────┴────────┐          ┌───────┴────────┐
         │                │          │                │
         ▼                ▼          ▼                ▼
     ┌─────────┐     ┌─────────┐  ┌─────────┐     ┌─────────┐
     │ Primase │     │ Primase │  │ Primase │     │ Primase │
     └────┬────┘     └────┬────┘  └────┬────┘     └────┬────┘
          │                │           │              │
  Synthesizes RNA      Synthesizes RNA   Synthesizes RNA    Synthesizes RNA
     primers (5'→3')   primers (5'→3')   primers (5'→3')    primers (5'→3')
          │                │           │              │
          ▼                ▼           ▼              ▼
  ┌──────────────────────────────────────────────┐
  │          DNA Polymerase III                  │
  │ (Main enzyme for DNA synthesis extending RNA primer) │
  └──────────────────────────────────────────────┘
          │                │
   Leading Strand   Lagging Strand
     (Continuous)   (Discontinuous)
          │                │
          ▼                ▼
  Continuous synthesis  Synthesizes short segments
     in the 5' to 3'   called Okazaki fragments
          direction            in the 5' to 3' direction,
          using            but overall 3' to 5' template
          template strand         reading
          in 3' to 5'
                               │
                               ▼
                 ┌────────────────────────┐
                 │ RNA Primer Removal and │
                 │  Gap Filling           │
                 │  (DNA Polymerase I)    │
                 └────────────────────────┘
                               │
                               ▼
                 ┌────────────────────────┐
                 │ Fragment Joining       │
                 │ (DNA Ligase)           │
                 └────────────────────────┘

Additional Notes:
- The replication fork is the Y‐shaped region where the double‐stranded DNA is separated.
- \( \text{Helicase} \) unwinds the double helix, while the single-strand binding (SSB) proteins stabilize the unwound strands.
- \( \text{Primase} \) synthesizes short RNA primers that provide a free 3΄-OH group.
- \( \text{DNA Polymerase III} \) extends from these primers synthesizing new DNA continuously on the leading strand and discontinuously on the lagging strand (creating Okazaki fragments).
- \( \text{DNA Polymerase I} \) later replaces the RNA segments with DNA.
- \( \text{DNA Ligase} \) seals the nicks between the Okazaki fragments to ensure a continuous DNA strand.

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Extra Insights

While I can’t draw a diagram for you, I can paint a vivid picture! Imagine a long, twisted ladder—this is your DNA strand. During replication, the enzyme helicase acts like a zipper, unwinding the double helix. Next, DNA polymerase steps in, adding complementary nucleotides to each strand, making copies like a molecular photocopier. Single-strand binding proteins keep the unwound strands apart, and primase lays down RNA primers to get everything started. Finally, ligase comes in to stitch the fragments together, ensuring the new strands are seamless and ready for action!
Now, let’s explore the intrigue of DNA replication! Did you know that our DNA goes through this miraculous process whenever our cells divide, ensuring that each new cell has an identical copy? It’s a remarkable and finely tuned system, evolved over billions of years, with mechanisms in place to correct mistakes—because even nature prefers quality control! And if you thought that a simple sequence of nucleotides was just for us humans, think again! Every living organism replicates its DNA using an incredibly similar process, showcasing the unity in the diversity of life.

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